• Brassica rapa 300k Microarray v2.0

  • The Microarray was designed from 47,548 unigenes. Among these, 32,395 genes were from representative sequences with cDNA/EST supports and 17,458 genes were predicted without cDNA/EST supports.
    Seven 60-nt long probes were designed from each gene starting 60 bp ahead the stop codon and with shifting 15 bp between probes so 7 probes cover 150 bp in the 3' region of the gene. In total, 331,384 probes were extracted. Genes from chloroplast (6), mitochondria (103) and selection marker genes such as gfp, gus, hyg, bar, and kan are included. The average size of probe is 60-nt long with adjusting its Tm value 75 to 85℃. The microarray was manufactured at NimbleGen inc (http://www.nimblegen.com/). Random GC probes (40,000) to monitor the hybridization efficiency and four corner fiducial controls (225) were included to assist with overlaying the grid on the image.

  • Hybridization

  • Genes on microarray


Brassica rapa 300k Microarray