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Basic vector construction :
¿î¹Ýü¸¦ Á¦ÀÛÇØ µå¸®´Â ¼ºñ½º ÀÔ´Ï´Ù.
Destination vector construction :
ÇüÁúÀüȯ¿ë ¶Ç´Â ´Ü¹éÁú ¹ßÇö¿ë ÃÖÁ¾ ¿î¹Ýü¸¦ Á¦ÀÛÇØ µå¸®´Â ¼ºñ½º ÀÔ´Ï´Ù.
TA cloning :
PCR »ê¹°À» T-vector¿¡ »ðÀÔÇÏ¿© ¼±º°µÈ cloneÀ» sequenceing ÇÏ¿© PCR »ê¹°ÀÇ Á¤º¸¸¦ Á¦°øÇÏ´Â ¼ºñ½ºÀÔ´Ï´Ù.
Transformation of Agrobacterium :
À¯ÀüÀÚ°¡ µµÀÔµÈ ¿î¹Ýü¸¦ Agrobacterium¿¡ ÇüÁúÀüȯ ½ÃÅ°´Â ¼ºñ½º ÀÔ´Ï´Ù.
- total RNA preparation
- RNA purification and concentration
- DNA preparation
- PCR product purification
- Gel extraction
- Cell stock
- E.coli transfomation
- Primer Á¦ÀÛ
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