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Gene cloning |
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:: Basic vector construction
¿î¹Ýü¸¦ Á¦ÀÛÇØ µå¸®´Â ¼ºñ½º ÀÔ´Ï´Ù.
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:: Destination vector construction
ÇüÁúÀüȯ¿ë ¶Ç´Â ´Ü¹éÁú ¹ßÇö¿ë ÃÖÁ¾ ¿î¹Ýü¸¦ Á¦ÀÛÇØ µå¸®´Â ¼ºñ½º ÀÔ´Ï´Ù.
Gene cloning
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1. For Over-expression [On Top] |
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Name |
T-DNA Structure |
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pMJ101 |
BR- MAR-[P OsCc1-(attR1-Cm-ccdB-attR2)-T PinII]-[P 35S-Bar-T nos]-MAR-BL |
MAP |
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pMJ102 |
BR- MAR-[P OsCc1-(attR1-Cm-ccdB-attR2)-T nos]-[P 35S-Hyg-T 35S]-MAR-BL |
MAP |
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pMJ103 |
BR- MAR-[P Glb-(attR1-Cm-ccdB-attR2)-T PinII]-[P 35S-Bar-T nos]-MAR-BL |
MAP |
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pMJ104 |
BR- MAR-[P rbcS-(attR1-Cm-ccdB-attR2)-T PinII]-[P 35S-Bar-T nos]-MAR-BL |
MAP |
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pMJ105 |
BR- MAR-[P Ai-(attR1-Cm-ccdB-attR2)-T nos]-[P 35S-hyg-T 35S]-MAR-BL |
MAP |
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2. For Post Transcriptional Gene Silencing/RNAi |
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Name |
T-DNA Structure |
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pMJ201 |
BR-MAR - [P OsCc1-(attR1-Cm-ccdB-attR2) - intron(SD,Amp,SA) - (attR2-Cm-ccdB-attR1) - T PinII] - [P 35S-Bar-T nos] - MAR-BL |
MAP |
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pMJ202 |
BR-MAR - [P OsCc1-(attR1-Cm-ccdB-attR2) - intron(SD,Amp,SA) - (attR2-Cm-ccdB-attR1)-T nos] - [P 35S-Hyg-T 35S] - MAR-BL |
MAP |
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:: TA cloning
PCR »ê¹°À» T-vector¿¡ »ðÀÔÇÏ¿© ¼±º°µÈ cloneÀ» sequenceing ÇÏ¿© PCR »ê¹°ÀÇ Á¤º¸¸¦ Á¦°øÇÏ´Â ¼ºñ½ºÀÔ´Ï´Ù.
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- ½Ã·áÁغñ :
- plasmid ³óµµ 100ng/ul, ÇÊ¿ä·® 50ul
- plsmidÀÇ sequence Á¤º¸(txt, hwp, docÈÀÏ·Î)
- ¼Ò¿ä±â°£ :
- ½Ã·áÀÇ sequence È®ÀÎ ÈÄ 2-6ÁÖ
- Á¦°øµÇ´Â µ¥ÀÌÅÍ :
- DATA sheet
- ¿Ï¼ºµÈ ¿î¹Ýü DNA¿Í E.coli cell stock
- °ü·Ã¼ºñ½º
- DNA preparation
- PCR product purification
- Gel extraction
- E.coli transfomation
- Primer Á¦ÀÛ
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:: Transformation of Agrobacterium
À¯ÀüÀÚ°¡ µµÀÔµÈ ¿î¹Ýü¸¦ Agrobacterium¿¡ ÇüÁúÀüȯ ½ÃÅ°´Â ¼ºñ½º ÀÔ´Ï´Ù.
- Agrobacterium Á¾·ù :
LBA4404(pSB1), GV3101, EHA101, EHA105
- ½Ã·áÁغñ :
- ¿î¹Ýü ³óµµ 100ng/ul, ÇÊ¿ä·® 30ul
- ¿î¹ÝüÀÇ sequence¿Í Map Á¤º¸(txt, hwp, docÈÀÏ·Î)
- ¼Ò¿ä±â°£ :
- ½Ã·áÀÇ sequence È®ÀÎ ÈÄ 2-3ÁÖ
- Á¦°øµÇ´Â µ¥ÀÌÅÍ :
- DATA sheet
- Agobacterium cell stock
- ½Ã·áÁ¢¼ö :
¼¿ï, ¼ö¿ø ±×¸®°í ¿ëÀÎ Áö¿ªÀÎ °æ¿ì¿¡´Â ´ç»ç Á÷¿øÀÌ Á÷Á¢ ¹æ¹®ÇÏ¿© ½Ã·á¸¦ ¼ö°ÅÇϸç,
±× ¹ÛÀÇ ±âŸ Áö¿ªÀÎ °æ¿ì¿¡´Â ÅÃ¹è µîÀ» ÀÌ¿ëÇÏ¿© Dry-ice¸¦ ä¿ö ³Ãµ¿»óÅ·Πº¸³»Áֽñ⠹ٶø´Ï´Ù.
(Àüȳª À̸ÞÀÏÀ» ÀÌ¿ëÇÏ¿© ¹æ¹®Á¢¼ö¸¦ ½ÅûÇÏ½Ã¸é µË´Ï´Ù.)
¢Á º¸³»½Ç °÷ :
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¢Á °ßÀû ¹× Service ¹®ÀÇ :
Tel. (031-330-6193) Fax. (031-321-6355)
E.mail service@ggbio.com
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